Overexpression of Tff3 improves glucose tolerance in DIO mice.

<p>Diet-induce-obesity (DIO) mice were injected with Ad-GFP or Ad-Tff3. Mice were sacrificed for following analysis 5 days after infection. (A-B) Quantitative real-time PCR showing mRNA levels of Tff3 and gluconeogenic genes (G6pc, PEPCK and PGC-1α) in livers of ob/ob mice treated as above in the fasted states (n=5). (C) Western blot analysis of anti-PGC-1α protein levels in livers of DIO mice treated as described in (A-B). (D) Blood glucose levels in the same mice as in (A-B) (n=5). (E-G) Glucose tolerance tests (GTT) (E), insulin tolerance tests (ITT) (F) and pyruvate tolerance experiment (PTT) (G) in Ad-GFP or Ad-Tff3-injected DIO mice were performed 5 days after infection (n=5). Data are mean ±SEM. *P < 0.05.