Overexpression of Tff3 reduces the blood glucose level and improves glucose tolerance in db/db mice.

<p>db/db mice were injected with Ad-GFP or Ad-Tff3. Mice were sacrificed for following analysis 5 days after infection. (A-B) Quantitative real-time PCR showing mRNA levels of Tff3 and gluconeogenic genes (G6pc, PEPCK and PGC-1α) in livers of db/db mice treated as above in the fasted states (n=5). (C) Western blot analysis of anti-PGC-1α protein levels in livers of db/db mice treated as described in (A-B). (D) Blood glucose levels in the same mice as in (A-B) (n=5). (E) Blood Glucose levels were measured in another groups of mice injected with Ad-GFP or Ad-Tff3 in different day (4, 6, 8 days) after infection. (F-H) Glucose tolerance tests (GTT) (F), insulin tolerance tests (ITT) (G) and pyruvate tolerance experiment (PTT) (H) in Ad-GFP or Ad-Tff3-injected db/db mice were performed 5 days after infection (n=4). Data are mean ±SEM. *P < 0.05, **P < 0.01.