The ERM kinase MAP4K4 localizes to leading and trailing edges of migrating TaH12810 cells.

<p><b>A</b>) IF microscopy analysis of ERM and MAP4K4 protein localization in cells migrating in matrigel using anti-ERM and anti-MAP4K4 antibodies. Magnifications are 4x of boxed area and highlight MAP4K4 accumulation in membrane blebs. Arrows indicate direction of migration. <b>B</b>) Time-lapse microscopy of TaH12810 cells expressing either YFP-ezrin (movies S11 & S13) or EGFP-MAP4K4 (movies S9 & S10). LA-mCherry was used to visualize leading edge actin dynamics (movie S12). Cells migrate in matrigel either in saltatory (upper) or tunneling (lower) mode (from left to right: grey scale, GFP or YFP fluorescence, overlay). Dotted line indicates cavity boundary, stars indicate leading edge of cells.