Repopulation of AdTk/GCV treated mice livers with human hepatocytes and analysis of lipid accumulation.

<p>A) Mice infected with AdTk and treated with GCV were transplanted with human hepatocytes, animals sacrificed at diverse time points after transplantation and liver sections were immunostained with hCK18 antibody. Liver samples were obtained from 9 different independent experiments and at least two stained liver sections from 6 mice belonging to three independent studies were analyzed at each time point. Obtained images were examined, quantified and represented as the percentage of hepatic section replaced by human hepatocytes (** <i>p</i>< 0.01, *** <i>p</i>< 0.001, two-tailed Student’s <i>t</i>-test). Presented images show human mouse chimeric livers collected at week 14 after transplantation. B) Frozen section of a chimeric liver containing human hepatocytes stained with fat red for detection of lipids (pink colour). C) A serial section of the same liver sample immunostained for hCK18 identifying human hepatocytes. Original magnification x20.