Deletion of <i>Gfi1</i> and <i>Gfi1b</i> results in increased V(D)J recombination in B cells and pDCs.

<p>(<b>A</b>) Flow cytometric analysis of VEX expression in B cells derived from the bone marrow of 2 individual <i>Gfi1</i>^<i>f/f</i><i>; Gfi1b</i>^<i>f/f</i><i>; ERCre, SVEX</i> mice cultured in 5ng/ml IL-7 for 7 days (solid line), untreated (top panel) and treated (bottom panel) with tamoxifen (4-OHT). Cells were gated on B220⁺. Shaded histogram denotes background fluorescence from <i>Gfi1</i>^<i>f/f</i><i>; Gfi1b</i>^<i>f/f</i><i>; ERCre</i> cells. Vertical axis ('% of max') indicates a scale of relative cell numbers with the median value set as 100%. (<b>B</b>) Flow cytometric analysis of VEX expression in pDCs derived from bone marrow from 2 individual <i>Gfi1</i>^<i>f/f</i><i>; Gfi1b</i>^<i>f/f</i><i>; ERCre, SVEX</i> mice cultured in 25ng/ml Flt-3L for 8 days (solid line), untreated (top panel) and treated (bottom panel) with tamoxifen. Cells were gated on B220⁺ CD11c⁺ cells. Shaded histogram denotes background fluorescence from <i>Gfi1</i>^<i>f/f</i><i>; Gfi1b</i>^<i>f/f</i><i>; ERCre</i> cells. Vertical axis ('% of max') indicates a scale of relative cell numbers with the median value set as 100%. All data are representative of at least three independent experiments. (<b>C</b>) Dot plot showing percentage of SVEX+ cells in Flt-3L cultures untreated (-4-OHT) and treated (+4-OHT) with tamoxifen. p-value was calculated using the two-tail paired Student’s <i>t</i>-test.