Deletion of <i>Gfi1</i> and <i>Gfi1b</i> results in increased expression of <i>Rag</i> in pDCs.

<p>(<b>A</b>) Quantitative RT-PCR analysis of <i>Rag1</i> transcript levels in sorted B220⁺ CD11c⁺ pDCs derived from 3 individual <i>Gfi1</i>^<i>f/f</i><i>; Gfi1b</i>^<i>f/f</i><i>; ERCre</i> mice untreated and treated with tamoxifen (4-OHT). Values are normalized to <i>Hprt1</i> transcript abundance. p-values were calculated with the two-tail Student’s <i>t</i>-test. * denotes p < 0.005. (<b>B</b>) Genotyping PCR of <i>Gfi1</i> and <i>Gfi1b</i> loci from sorted B220⁺ CD11c⁺ pDCs derived from 4 individual <i>Gfi1</i>^<i>f/f</i><i>; Gfi1b</i>^<i>f/f</i><i>; ERCre</i> mice untreated and treated with tamoxifen (4-OHT). Genomic DNA was isolated and subjected to PCR analysis using primers that detect wildtype (WT), floxed and deleted alleles of <i>Gfi1</i> and <i>Gfi1b</i>. PCR products were run on 1% agarose gel and visualized with ethidium bromide. All data are representative of at least two independent experiments.