<i>Meloe</i> silencing in melanoma cells with a short interfering RNA (siRNA) localized upstream of MELOE-2 ORF.

<p>A. M88 melanoma cells were transfected with 50(control si) or siRNA hybridizing 55 bp upstream of MELOE-2 ORF (<i>meloe</i> si) along with a GFP reporter plasmid. Relative expression levels of <i>meloe</i> mRNA were evaluated on GFP+ sorted cells by qPCR with MELOE-1 primers. B. TNF secretion by MELOE-1 (M170.48) and MELOE-2 (M170.51) T cell clones in response to GFP+ sorted M88 cells transfected with the different siRNAs. Co-cultures with specific clones were performed at 1∶2 clone to melanoma cell ratio and response of each clone was assessed by TNF-α intracellular staining.