Modulation of the innate immune response sensors in CHIKV infected U-87 MG cells.

<p><b>A</b>: <b>Semi-quantitative RT-PCR</b>. Total RNA from the infected and uninfected cells was isolated 24h p.i and was subjected to semi-quantitative RT-PCR as described in the methods. The PCR band intensities of the target cDNA amplicons, determined from densitometric analysis after agarose-gel electrophoresis, were initially normalised to that of corresponding β-Actin amplicon, and the fold change in expression was calculated relative to the gene expression in uninfected controls at the respective time-points. Values are Mean ± SD from three independent biological experiments each done in duplicate. ‘*’ indicates significant modulation with respect to the basal level (0h) gene expression. <b>B</b>. <b>Western blot and quantitation by densitometric analysis</b>. Total cell lysate from the infected and uninfected cells was isolated 24h p.i and 50-100µg of total protein was subjected to SDS-PAGE and western blot as described in the methods. The band intensities of the targets, determined from densitometric analysis, were initially normalised to that of corresponding β-Actin, and the fold change in expression was calculated relative to the protein expression in uninfected controls at 24h p.i. Values are Mean ± SD from three independent experiments. ‘*’ indicates statistically significant (p<0.05) modulation with respect to the expression in the uninfected cells.