Genetic regulation of HH signaling modulates hTERT expression in human colon cancer cells.

<p><b><i>A:</i></b> HT29, <b><i>B:</i></b> HCT116 cells were transiently transfected with GLI3R (myc-tagged) for 48 hr or 72 hr, respectively. Steady state levels of GLI1, GLI2 and hTERT were determined by Western analysis represented as densitometry of the blots in <b><i>A</i></b> with one representative hTERT blot (inset). Expression of GLI3R was determined using anti-myc antibody. <b><i>C:</i></b> pBP empty vector (V) or full length GLI1 cDNA in pBP (GLI1) or GLI2ΔN in pBP (GLI2ΔN) was stably expressed into HT29 cells, and cells were cultured for 10 passages. Steady state levels of GLI1, GLI2 and hTERT were measured by Western blot. HSP90α/β was used as a loading control. *p<0.0001.