Structure of 4E10 free of bound antigen.

<p>(<b>A</b>) Superposition of the three HCDRs from bound and unbound b12 (3RU8.pdb <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003639#ppat.1003639-Azoitei1" target="_blank">[104]</a>, 1HZH.pdb <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003639#ppat.1003639-Saphire1" target="_blank">[110]</a> and 4E10 (2FX7.pdb <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003639#ppat.1003639-Cardoso1" target="_blank">[9]</a>, 4LLV.pdb) are shown in a B-factor putty representation (b12, blue: bound; cyan: unbound. 4E10, pink: bound, yellow: unbound). (<b>B</b>) Superposition of residues from the 4E10 epitope binding site and HCDR1 and 3 from bound (semi-transparent molecular surface in pink; 2FX7.pdb <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003639#ppat.1003639-Cardoso1" target="_blank">[9]</a>) and unbound (mesh molecular surface in yellow; 4LLV.pdb) 4E10 Fv are shown; HCDR1 and 3 are shown in cartoon representations with side-chains of key residues shown in licorice-stick representations and labeled. The molecule is oriented so that the V_L domains are to the left and the V_H domains to the right. Molecular images were generated with MacPyMOL <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003639#ppat.1003639-DeLano1" target="_blank">[109]</a>.