PI3-K/Akt signaling is involved in cordycepin-suppressed β-catenin.

<p>U937 cells were treated with cordycepin (100 µM) combined with/without (A) compound c (1 µM), (B) rapamycin (10 µM) and (C) Ly-294002 (20 µM) for 4 hrs. Expression level of β-catenin, Akt phosphorylated Akt (Ser473) were determined by Western blot analysis. Actin was used as loading control. (D) U937 cells were treated with cordycepin (100 µM) for 5, 60 and 240 min. Expression level of phosphorylated Akt (Ser473), Akt, phosphorylated GSK-3β (Ser9) and GSK-3β were determined by Western blot analysis. Actin was used as loading control. (E) A schematic model illustrating the role of cordycepin in suppressing β-catenin in leukemia cells.