NB cells with DCL knockdown proliferate less in an energetic challenged environment.

<p>(A-D) Cell proliferation in Dox-inducible NB cell lines in the presence of low glucose (A) or high glucose (B) medium and treated with doxycycline (Dox) or vehicle (Veh) for 72 hours. Cell proliferation in shDCL-2 (C) and shDCL-3 (D) cells 72 hours after starting the treatment with either Dox or Veh and in the presence of low or high glucose medium. (E) Ratio in cell proliferation between Dox-inducible NB cells that grew in low and high glucose medium. (F) Cell proliferation in shDCL-2 and shDCL-3 cells grown in high glucose medium, treated with Dox or Veh for 72 hours and subsequently transfected with empty plasmid or DCL plasmid to recover DCL expression. NC, negative control Dox-inducible NB cell lines. shDCL-2 and shDCL-3, Dox-inducible NB cell lines that express shRNA against DCL. Error bars, s.e.m. *, <i>P</i><0.05; **, <i>P</i><0.01.