DCL knockdown in Dox-inducible NB tumors correlates with less proliferation and enhanced apoptosis.

<p>(A) DCL and α-tubulin expression in Dox-inducible tumors from nude mice treated with doxycycline- (Dox) or vehicle (Veh)-diet. (B) DCL expression normalized to α-tubulin. (C) Immunostaining of Ki67 (green) positive cells in the Dox-inducible NB tumors. (D) Average of positive Ki67 cells per high-power field (HPF). Immunostaining (E) and quantification (F) of cleaved caspase-3 positive cells (green) per HPF. Images (C and E) are representative of the average expression of each group. For each section, 5-10 different fields were analyzed. NC, negative control Dox-inducible NB tumors. shDCL-2 and shDCL-3, Dox-inducible NB tumors that express shRNA targeting DCL. Blue, Hoechst staining. Red, α-tubulin staining. Arrows, examples of Ki67 positive cells (C) or cells with cleaved caspase-3 (E). Error bars, s.e.m. *, <i>P</i><0.05. Scale bars, 25 µm (C) or 50 µm (D).