DCL knockdown in Dox-inducible NB cells results in less NB proliferation.
- Carla S. Verissimo (463419)
- Rachel Elands (463420)
- Sou Cheng (463421)
- Dirk-Jan Saaltink (463422)
- Judith P. ter Horst (463423)
- Maria N. Alme (463424)
- Chantal Pont (463425)
- Bob van de Water (108410)
- Bjarte Håvik (170131)
- Carlos P. Fitzsimons (463426)
- Erno Vreugdenhil (463427)
Publication date
September 2013
DOIAbstract
<p>(A) shRNA expression in the different NB cell lines treated with doxycycline (Dox) or vehicle (Veh) for 72 hours. (B) Western blotting results of DCL and α-tubulin expression in Dox-inducible NB cells treated for 72 hours with doxycycline (Dox) or vehicle (Veh). (C) Quantification and normalization of DCL expression to α-tubulin. (D) Cell proliferation 72 hours after starting adding Dox or Veh to the growth medium. N1E-115 cells, mouse NB cell line used to develop the Dox-inducible NB cell lines. NC, negative control Dox-inducible NB cells. shDCL-2 and shDCL-3, Dox-inducible NB cell lines that express shRNA against DCL. RQ, relative quantification. Error bars, s.e.m. **, <i>P</i><0.01; ***, <i>P</i><0.001.</p
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