TNFα is internalized into RhoB-positive vesicles.

<p>(A) Cells were stained for RhoB before and after stimulation with TNFα for 4 hours (upper panels) and before and after proteasome inhibition with MG132 for 1 hour (lower panels); (B) Cells were stimulated with TNFα for 4 h and stained for RhoB (green) and EEA1 (red). Arrows point to vesicles positive for both proteins; (C) Cells were incubated at 4°C with biotin-TNFα and FITC-streptavidin (green) and transferred to 37°C for 30 min to allow internalization. Following fixation/permeabilization, cells were stained for RhoB (red). A magnification of the area within the box is shown on the right. Arrows point to vesicles where TNFα colocalises with RhoB. Bars: 10 µm.