Interaction between ¹⁵N, ¹³C labeled XD and unlabeled N_TAIL.

<p>(A) Superposition of ¹H-¹⁵N HSQC spectra of XD containing increasing amounts of N_TAIL: 0% (red), 71% (green) and 416% (blue). The spectra were obtained at a ¹H frequency of 600 MHz and 298 K. (B) Combined ¹H and ¹⁵N chemical shift difference () between the resonances in the ¹H-¹⁵N HSQC spectrum of isolated XD and the spectrum with 416% N_TAIL. Gray vertical shading indicates residues for which the NMR resonances disappear upon addition of N_TAIL. Chemical shift changes above the horizontal dashed line are considered significant. (C) Surface representation of the crystal structure of XD displaying residues experiencing large chemical shift changes upon addition of N_TAIL (orange). The orientation of XD is the same as shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003631#ppat-1003631-g003" target="_blank">Figure 3</a>. (D) Surface representation of the crystal structure of XD displaying the location of hydrophobic (beige), negatively charged (red) and positively charged (blue) residues. (E) Helical wheel representation of the MoRE of N_TAIL encompassing residues 472–489 with the same color-coding as in (D). (F) Surface representation of the MoRE of N_TAIL assuming that the MoRE adopts an α-helical conformation.