Ed-MeV exploits autophagy to replicate.

<p>(A) HeLa cells were treated with the indicated si-RNA for 48 h and infected with Ed-MeV (MOI 3). One, two or three days post infection infectious viral particles were titrated by plaque assay. (B–D) HeLa cells were treated or not with 125 nM rapamycin (Rapa) or 50 µM chloroquine (CQ) and infected with Ed-MeV (MOI 1 (B) or MOI 0.1 (C–D)). Three days post infection infectious viral particles were titrated by plaque assay (B) or 48 h post-infection, cells were lysed and MeV-N (C), MeV-P (D) expression were detected by western blot. (C–D) Representative results are shown and are accompanied by a graph representing the intensity of MeV-N (C) or MeV-P (D) expression over actin, normalized to the non-infected condition. For each experiment, error bars, mean ± SD of three independent experiments. Student's t test; ***p<0.005; **p<0.01; *p<0.05; #p>0.05.