Stat3 activation regulates TF expression at mRNA level in lung cancer cells.

<p>(A) CL 1-5, H1650 and AS2 lung adenocarcinoma cells were treated with the JAK inhibitor, INC424 (2.5 µM), TF mRNA levels were analyzed by RT-PCR. (B) CL 1-0 cells were treated with 5-AZAdC (5 µM) everyday for three days and mRNA was prepared for examination of TF expression. (C) mRNA of PC14PE6/AS2-Vec, S3C(1), S3C(2) cells was extracted and subjected to RT-PCR analysis using TF and GAPDH primers. (D) PC14PE6/AS2 cells were transiently transfected with TF promoter reporter gene and active form Stat3 (Stat3C) plasmids or vector control plasmid (Vec). The luciferase activity was determined by luciferase assay system 24 hr after transfection.