Blockage of TF expression in PC14PE6/AS2 cells decreased ability of vascular permeability and ascites formation.

<p>(A) Gross appearance of nude mice showed abdominal distension with dark fluid after 21 days in mouse injected with siVec(1) cells, but not in two other groups of mice injected with siTF(3) and siTF(8) cells. (B) Serum-free culture supernatants of tumor cells were harvested for the Miles assay. Nude mice were intravenously injected with Evans blue dye. After 10 min, 50 µl of test samples (from PC14PE6/AS2, siVec(1), siTF(3), or siTF(8) cells) were injected into the dermis of the mice. The subdermis was harvested and photographed after 30 min. Dye leakage areas were indicated by dashed black circles and quantified (C) (n=3).