Blockage of Stat3 activation decreased TF activity and tumor formation <i>in vivo</i>.

<p>(A) Before injecting the cells into nude mice, Vec(1), Vec(2), S3C(1) and S3C(2) cells were harvested and the expression levels of TF and Stat3 were evaluated by Western blot analysis. (B) The same cells as (A) were harvested and TF activity assay were performed to measure the ability to initiate the coagulation cascade. (C) Nude mice were subcutaneously injected with 1 × 10⁶ cells of the vector control clone Vec(1) or dominant-negative Stat3 clones, S3D (1) and S3D(2). Tumor volume was measured weekly. The values of tumor volume represent an average of five mice for each group. *Significantly different from vector controls (P < 0.05) at indicated times (D).