qRT-PCR analysis of mRNA levels.
- Leah C. Byrne (463903)
- Fakhra Khalid (463904)
- Trevor Lee (463905)
- Emilia A. Zin (463906)
- Kenneth P. Greenberg (463907)
- Meike Visel (260936)
- David V. Schaffer (186688)
- John G. Flannery (258472)
Publication date
September 2013
DOIAbstract
<p>Quantitative RT-PCR performed on mRNA harvested from KillerRed and GFP-injected eyes, three months after irradiation, revealed that levels of GS (64% of WT), CRALBP (65%), Rhodopsin (76%), and GNAT2 (82%) were reduced in KillerRed-injected eyes compared to GFP-injected eyes or WT eyes. Levels of GFAP (205% of WT) increased, while levels of VEGF (98%) were equal to WT at the time point measured. A one-way ANOVA with a Tukey multiple comparison test was used to determine significance (*=P<0.05; ** = P<0.01).</p
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