The impact of TROP2 expression on cell apoptosis.

<p>A. Down-regulation of TROP2 induced cell apoptosis in Siha and CaSki cells. Cells were harvested at 48 h after transfection, followed by apoptosis assay using the annexin V-FITC apoptosis detection kit. Cells in the right lower and upper quadrants are consider as early and late apoptosis respectively, in the left upper quadrants are considered as dead cells. The results were analyzed by FlowJo software. B. Enforced expression of TROP2 significantly inhibited cell apoptosis C. Western blot analysis showed that down-regulation of TROP2 reduced the expression of bcl-2 and increased the expression of bax in Siha and CaSki cells. D. Enforced expression of TROP2 in HeLa and C33A cells increased the expression of bcl-2 and reduced the expression bax. These data are expressed as mean ± SD of three independent experiments, *p<0.05, **P<0.01.