Subcellular distribution of Cav1, PAF-R and eNOS in mouse lung ECs.

<p>Representative micrographs of Cav1, PAF-R and eNOS immunolocalization in mouse PAECs (<b>A</b>) and MVECs (<b>B</b>); Bars: 22 µM. n = 12. (<b>C</b>) Overall chart of subcellular fractionation using silica isolation procedure. (<b>D</b>) Blots illustrating the distribution of Cav1, PAF-R and eNOS in the sub-cellular fractions isolated as in C. TH = total amount of protein obtained by solubilization of coated monolayers (unfractioned); BM - basolateral plasma membranes, APM - apical plasma membranes; IMF1 - internal membranes that sediment at low gravitational fields as well as mostly of the cytosolic proteins; IMF2 - endoplasmic reticulum and nuclei; n = 8. (<b>E</b>) Densitometric analyses of the gels shown in D; * P < 0.05, ✠ P < 0.001.