Comparison of the viral yields produced using lipofectamine 2000 or PLGA/bPEI-DNA nanoparticles.

<p>HEK293FT cells were co-transfected with pMX-GFP, gag/pol, and VSV-G, and the culture supernatants were harvested at 48 h after transfection. (A) human adipose tissue-derived mesenchymal stem cells were incubated with the viral supernatant (1:1, v/v), and fluorescence and bright field images are shown. n=5. (B, C) NIH 3T3 cells were incubated with the viral supernatant with or without serial dilution (x10, x100, x1000). Fluorescence images and flow cytometry analysis of cells at 48 h after incubation are shown. Scale bars represent 100 µm (*, p < 0.05 by Student’s t-test). Virus infection was tested in 60 mm plates. n=3.