Lung index and viral titer of mice.

<p>Mice were inoculated intranasally with 10^5.0 and 10^7.9 EID₅₀ of H1N1 influenza virus respectively on day 0 and gavaged with 5% ethanol vehicle control from day -1 to day 6 (N=12) or day 5 PI (N=15), or inoculated with virus on day 0 and gavaged with 110 mg/kg <i>H</i>. <i>perforatum</i> extract in 5% ethanol (N=12 for low viral titer study and N=15 for high viral titer study), or not inoculated with virus and gavaged with 5% ethanol (N=6 for 6-day study and N=5 for 5-day study). At the end of the study, the mouse lungs were weighed and used to calculate lung index (A and C) by histopathology, shown as Mean ± SEM. Lung viral titer (B and D) was measured using qRT-PCR, with log values for relative viral titer shown as Mean ± SEM. Values without the same label are different from each other statistically (p<0.05, a > b > c).