Plot of canonical discriminant scores for individual mice in each group according to Low, Medium and High frequency normal behaviour data from analysis with HCS (Automated).

<p>Groups are identified as in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075948#pone-0075948-g001" target="_blank">Figure 1</a> legend; the axis labels show the significance of each Function and its respective percentage contribution to between-group variation. Although activity changes showed significant separation of the surgery and control groups (Function 1; P<0.001), compared with the results of pain scoring there was a lack of differentiation according to whether mice were treated with saline or buprenorphine. Low frequency measures were: ‘Jumping’ and ‘Come Down’; Medium frequency: ‘Rear Up’, ‘Walk Left’ and ‘Walk Right’; High frequency: ‘ComeDown from and to Partially Reared’ (2 behaviours), ‘RearUp from and to Partially Reared, ‘Remain Low’, Remain Partially Reared’, ‘Sniff’ and ‘Walk Slow’. (n = 8 per group).