Comparative distribution of eukaryotic sequences in Great Barrier Reef coral holobionts.

<p>(A) Abundance of sequences belonging taxonomic groups of Eukaryotes in corals using three tag encoded 454 amplicon pyrosequencing assays targeting eukaryote small-subunit rRNA gene assays (Euk1, Euk2, Euk3). Relative position of the universal primer pairs utilised Euk1-3 assays is schematically shown on a complete small-subunit rRNA gene (inset right). (B) Venn diagram of sequence 97% sequence identity clusters shared between corals according to Euk1-3 assays. Number of sequences used to calculate Venn diagram within 97% clusters (>1 sequence) obtained using Euk1-3 assay and DNA indicated on the left (colour coded according to coral DNA). Total number of sequences recovered for each Euk1-3 (number in brackets on the right) and number sequences represented by >1 sequence (n). Coral species <i>Acroporapalifera</i> 42a, <i>Montipora</i><i>digitata</i> 60a, <i>Porites</i><i>cylindrica</i> 66-2 from Heron Island and <i>Seriatopora</i><i>hystrix</i> 176-2 from the One Tree Island.