Some photoswitching properties of Phanta and EGFP^C3Phanta.

<p>(A) The absorbance at 505 nm (λ_max^abs) for eCGP123 (triangle), Phanta (circle) and Dronpa (square) was determined for proteins in solution in the well of a microtitre plate after exposure first to photoswitching cyan light, then photoswitching violet light. The change from cyan to violet photoswitching illumination occurred after the symbol circled in red. (B) A solution of Phanta in the well of a microtitre plate was subjected to repeated cycles of photoswitching by exposure to defined periods of cyan or violet photoswitching light provided by an LED. Absorbance (505 nm; ON-state) is shown for each cycle after exposure to cyan light (filled bar) or violet light (open bar). The change in absorbance for each photoswitching cycle is shown (filled squares). Data plotted as the average of three separate experiments ± s.e.m. are shown.