<i>2mit</i> knockdown in adult flies.

<p>(A) <i>2mit</i> mRNA relative levels (mean ± SEM) in the dissected brains of three independent <i>elavGal4>2mit</i>KD (<i>6.1</i>; <i>16.2</i>; <i>61.1</i>) and <i>w</i>¹¹¹⁸ controls. Plot of 6 replicates. F_3,20= 1571, p<0.0001; Newman-Keuls <i>post-hoc</i> test: each <i>elavGal4>KD</i> line <i>vs</i> <i>w</i>¹¹¹⁸ controls: p< 0.001. (B) Courtship Indices in sham (white bars) and conditioned (black bars) males for <i>elavGal4>2mit</i>KD (<i>6.1</i>; <i>16.2</i>; <i>61.1</i>) and appropriate controls [<i>elavGal4>+</i>; +> <i>2mit</i> KD (<i>6.1</i>; <i>16.2</i>; <i>61.1</i>)]. Mann-Whitney U test showed no significant differences between the CIs of sham and conditioned males in all the <i>elavGal4>2mit</i> KD (<i>elavGal4</i>><i>2mit</i> KD^<i>6.1</i>.: p= 0.17; <i>elavGal4</i>><i>2mit</i> KD^<i>16.2</i>: p= 0.13; <i>elavGal4</i>><i>2mit</i> KD^<i>61.1</i>: p= 0.88). Significant differences between the CIs of conditioned and sham males were found in the control lines <i>elavGal4</i>> +; +> <i>2mit</i> KD^<i>61</i>. ; +> <i>2mit</i> KD^<i>16.2</i> ; +> <i>2mit</i> KD^<i>61.1</i> ; +> <i>2mit</i> KD^<i>61.1</i>. (C) Courtship Indices in sham (white bars) and conditioned (black bars) males for OK107<i>Gal4>2mit</i>KD (<i>6.1</i>; <i>16.2</i>; <i>61.1</i>), c772<i>Gal4>2mit</i>KD (<i>6.1</i>; <i>16.2</i>; <i>61.1</i>), MB247<i>Gal4>2mit</i>KD (<i>6.1</i>; <i>16.2</i>; <i>61.1</i>) lines, and the control lines OK107<i>Gal4>+</i>, c772<i>Gal4>+</i>, MB247<i>Gal4>+</i>. Mann-Whitney U test showed no significant differences between the CIs of sham and conditioned males in two out of three OK107<i>Gal4>2mit</i> KD lines (OK107<i>Gal4>2mit</i> KD^<i>16.2</i>: p= 0.18; OK107<i>Gal4>2mit</i> KD^<i>61.1</i>: p= 0.36), in all the c772<i>Gal4>2mit</i> KD flies (c772<i>Gal4</i>><i>2mit</i> KD^<i>61</i>. : p= 0.67; c772<i>Gal4</i>><i>2mit</i> KD^<i>16.2</i>: p= 0.72; c772<i>Gal4</i>><i>2mit</i> KD^<i>61.1</i>: p= 0.42) and in all the three MB247<i>Gal4>2mit</i> KD lines (M247<i>Gal4</i>><i>2mit</i> KD^<i>61</i>. : p= 0.88; MB247<i>Gal4</i>><i>2mit</i> KD^<i>16.2</i>: p= 0.12; MB247<i>Gal4</i>><i>2mit</i> KD^<i>61.1</i>: p= 0.27). Significant differences between the CIs of conditioned and sham males were found in the control lines OK107<i>Gal4>+</i>, c772<i>Gal4>+</i>, MB247<i>Gal4>+</i> and in the OK107<i>Gal4>2mit</i> KD^<i>6</i>1. transgenic line. In (B) and (C) data are expressed as mean ± SEM, with the number of tested flies indicated above each bar. The number of asterisks indicates the significance level: *: p < 0.05; **: p < 0.005; ***: p < 0.0001; ns: not significant.