Cdk5 kinase activity is required for in vitro hBMECs angiogenesis.

<p>Figure shows phase contrast images of in vivo time course analysis of cell spreading (A), cell migration (B), and the formation of tubule like structure in roscovitine (50 µM) treated (+) and untreated (-) cells (G). Graphs C and D report the computational analysis of the characterization of cell typologies during the progression of different phases of cell spreading in controls (C) and roscovitine treated cells (D), determined using Analyser_TM software. Compared to the controls (C), roscovitine (D) inhibited cell elongation and increased the time dependent cell distribution between early and late spreading. Graphs E and F show the number of living and dividing cells presents in the wounded monolayer area in controls (E) and with roscovitine treatment (F) determined using Analyser_TM software. (G) Representative time-lapse images showing the progression of tubule formation throughout the time course, in controls and in treated cells. Graphs H and I, show the relative quantitative evaluation of growth area in controls (H) and treated cells (I), determined by Analyser_TM software. (I) Roscovitine reduced the growth area after 10h, mainly destabilizing neo-forming cell junctions and integrating cell networks. (L) Representative images showing the inhibitory effect of roscovitine on sprout formation, compared to controls (-) and FGF-2 stimulated cells (positive control). The phase contrast images have been acquired as z-stacks and with automated focusing. Bars, 20 µm. Original magnification in L, x 200. Each experiment was performed in triplicate.