γ9⁺ T cells respond to restimulation with Heat-killed <i>B. pseudomallei</i> and CHMDAPP <i>ex vivo</i>.

<p>The effect of re-stimulation by CHMDAPP or heat-killed <i>B. pseudomallei</i> (HK B.ps.) on IFN-γ expression by γ9⁺ T-cells in cultured splenocytes. Fresh splenocytes were isolated from marmosets treated with CHMDAPP and IL-2 (n = 4), and group treated with IL-2 only (n = 4). Animals either received a single dose of CHMDAPP (day 0, at 2.5 mg/kg) and five doses of IL-2 (days 0, 1, 2, 3, 4 and 5, at 0.18 U×10⁶ per kg) or only received the five doses of IL-2 or received PBS only. These were cultured for 6 h with each stimulant and monitored for IFN-γ expression by flowcytometry. Fold induction was calculated by dividing the proportion of IFN-γ⁺ events at time of isolation by the proportion of IFN-γ⁺ events after <i>ex vivo</i> restimulation. Each data point represents the mean of 4 technical replicates and the line is the median of the 4 different animals. The lines are the medians and the P values are indicative of Bonferroni's post tests comparing parameters.