<p>Note: R = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, W = A/T, H = A/C/T, B = C/G/T, V = A/C/G, D = A/G/T, N = A/C/G/T. These primers were synthetized by BGI (Beijing, China).</p
<p>*PPO degenerate primer (R = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, W = A/T, H = A/C/T, B = C/G/...
<p><sup>a</sup>Capital letters indicate coding regions, underline indicates restriction enzyme sites...
A 1 kb molecular size standard was loaded on each side and in the middle of the gel. A total of 3 μl...
<p>The thick black line indicates the full-length <i>Of</i>RyR cDNA. S1–S17 represent the nucleotide...
<p>Note: N = A, G, C or T; R = A or G; Y = C or T; M = A or C; S = G or C; K = G or T; V = A, C or G...
<p>Primers designed for initial cloning of cDNA of glycoprotein hormone subunits and its leucine-ric...
a<p>The two primers (indicated as primer 1 and primer 2) were used for first PCR to synthesize the m...
<p>Primer sequences (no underline) are shown for PCR amplification, rapid amplification of cDNA ends...
<p>Key: F = Forward Primer, R = Reverse Primer.</p><p>For <i>CD44v</i>, the <i>CD44</i> P1 F primer ...
<p>PCR primers used for HepG2 <i>ΔTRIB1</i>::<i>Neo</i><sup><i>R</i></sup> clone construction.</p
<p>* RT primers were used for cDNA synthesis in each case; Reverse universal and Forward primers wer...
<p>qPCR = quantitative PCR.</p><p>bp = base pairs.</p><p><i>ori</i> = origin of replication.</...
<p>Primers used for 5’ flanking sequence cloning, deletion mutant construction, EMSA, mRNA construct...
<p>Primers starting with a D were degenerated primers to get the partial CDS of each gene. Primers s...
<p>Primers used for cloning, site-directed mutagenesis, and sequence analysis.</p
<p>*PPO degenerate primer (R = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, W = A/T, H = A/C/T, B = C/G/...
<p><sup>a</sup>Capital letters indicate coding regions, underline indicates restriction enzyme sites...
A 1 kb molecular size standard was loaded on each side and in the middle of the gel. A total of 3 μl...
<p>The thick black line indicates the full-length <i>Of</i>RyR cDNA. S1–S17 represent the nucleotide...
<p>Note: N = A, G, C or T; R = A or G; Y = C or T; M = A or C; S = G or C; K = G or T; V = A, C or G...
<p>Primers designed for initial cloning of cDNA of glycoprotein hormone subunits and its leucine-ric...
a<p>The two primers (indicated as primer 1 and primer 2) were used for first PCR to synthesize the m...
<p>Primer sequences (no underline) are shown for PCR amplification, rapid amplification of cDNA ends...
<p>Key: F = Forward Primer, R = Reverse Primer.</p><p>For <i>CD44v</i>, the <i>CD44</i> P1 F primer ...
<p>PCR primers used for HepG2 <i>ΔTRIB1</i>::<i>Neo</i><sup><i>R</i></sup> clone construction.</p
<p>* RT primers were used for cDNA synthesis in each case; Reverse universal and Forward primers wer...
<p>qPCR = quantitative PCR.</p><p>bp = base pairs.</p><p><i>ori</i> = origin of replication.</...
<p>Primers used for 5’ flanking sequence cloning, deletion mutant construction, EMSA, mRNA construct...
<p>Primers starting with a D were degenerated primers to get the partial CDS of each gene. Primers s...
<p>Primers used for cloning, site-directed mutagenesis, and sequence analysis.</p
<p>*PPO degenerate primer (R = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, W = A/T, H = A/C/T, B = C/G/...
<p><sup>a</sup>Capital letters indicate coding regions, underline indicates restriction enzyme sites...
A 1 kb molecular size standard was loaded on each side and in the middle of the gel. A total of 3 μl...
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