Ngb-IR in the penumbra and infarct volumes 24 hours after permanent middle cerebral artery occlusion.

<p>Ngb-IR in the cortex of a representative pMCAo operated Ngb-Tg mouse is shown in <b>A</b>. No increase in Ngb-IR intensity was observed in pMCAo operated Ngb-Tg mice when compared to sham-operated Ngb-Tg mice (<b>B</b>). The red line marks the penumbra. Black arrows exemplify Ngb-IR neurons and a black arrowhead a necrotic cell. No increase in Ngb expression is seen within nor adjacent to the penumbra area in the pMCAo operated Ngb-Tg animals compared to Tg-sham (<b>A</b>-<b>B</b>), which suggests no selective sparing of neurons expressing Ngb. Infarct volumes 24 hours after pMCAo estimated with 2D nucleator and Cavalierís principle is shown in <b>C</b>. The estimated infarct volume in cortex was significantly larger in WT Tg background mice (n = 5, total mean volume 3.5 mm³ SE±0.77 mm³) compared to Ngb-Tg (n = 7, total mean volume 1.7 mm³ SE±0.27 mm³) littermates. p<0.048. Also infarct volumes between the two different WT background strains differed significantly. The infarct volume of the WT mice with hsa1 background (n = 4 total mean volume 6.2 mm³ SE±0.26 mm³), was significantly larger compared to the infarct volume in the WT mice with Tg-background, p<0.032. Mean values are indicated by the lines and * denotes significance. Scale bar 50 µm.