Comparison of SL520 with its parent lines Koshihikari and Nona Bokra, and delimitation of the candidate genomic regions for <i>RFT1</i> and <i>Hd3a</i>.

<p>(A) Graphical genotype of SL520. The white and black regions denote the regions homozygous for Koshihikari and Nona Bokra alleles, respectively. (B) Flowering time of SL520, Koshihikari and Nona Bokra under short-day (SD:10L/14D), long-day (LD:14.5L/9.5D) and natural field (ND) conditions in Tsukuba, Japan in 2009. Each bar represents mean ± SD (n = 10). (C) Delimitation of the candidate genomic regions for <i>RFT1</i> and <i>Hd3a</i>. Left: Graphical genotypes of the <i>RFT1</i> and <i>Hd3a</i> regions in 10 lines, in which recombination occurred between InDel1 and RM7488. Right: Days to flowering under ND conditions. Each bar represents mean ± SD. The number of plants is indicated in parentheses. (D) Division of the <i>RFT1</i>/<i>Hd3a</i> region into three subregions. The 2.4-kb, 11.1-kb and 1.3-Mb candidate regions were defined by linkage analysis. The effect of the Nona Bokra allele on flowering time is shown below each region. The underlined 5-kb (chr. 6: 2,923,569–2,928,437; see Figure S4) fragment was used for complementation analysis.