Anti-nicotine antibody titer and function in mice.
- David C. Pryde (353590)
- Lyn H. Jones (465244)
- David P. Gervais (465245)
- David R. Stead (465246)
- David C. Blakemore (465247)
- Matthew D. Selby (465248)
- Alan D. Brown (465249)
- Jotham W. Coe (465250)
- Matthew Badland (465251)
- David M. Beal (465252)
- Rebecca Glen (465253)
- Yvonne Wharton (465254)
- Gavin J. Miller (465255)
- Phil White (465256)
- Ningli Zhang (465257)
- Michelle Benoit (465258)
- Karen Robertson (465259)
- James R. Merson (465260)
- Heather L. Davis (465261)
- Michael J. McCluskie (465262)
Publication date
October 2013
DOIAbstract
<p>Panel A: BALB/c mice (n = 12/gp) were immunized by IM injection with 10 µg of different nicotine Hapten-DT conjugates adjuvanted with Al(OH)<sub>3</sub> (40 µg Al<sup>3+</sup>) + CpG 24555 (50 µg) on days 0, 28 and 42. Plasma was collected on day 54 and anti-nicotine antibody levels determined by ELISA (Panel A). On day 56 animals received an IV injection of <sup>3</sup>H-nicotine (0.05 mg/kg) and plasma and brains collected. Panel B shows nicotine levels in plasma (ng-eq/mL), and Panel C shows nicotine levels in brain (ng-eq/g).</p
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