Infection of Daudi-CD4 cells with HIV.

<p>(A) Cells were infected with HIV (pNL4-3) and incubated with either HCV-positive patient serum (P1-P5) or HCV-negative serum (N1-N5) as described in Materials and Methods. Supernatant was harvested on days 3, 6, 9, and 12 and assayed for RT activity. Assay samples were spotted onto DEAE paper and visualized with autoradiography. (B) RT assay results quantified using densitometry. HIV RT activity is measured in arbitrary units. (C) To measure apoptosis resulting from exposure to HIV and HCV, cells were mock HIV-infected or infected with HIV then incubated with patient serum either positive or negative for HCV On days 3 and 5, cells were harvested, stained with annexinV (AV) and propidium iodide (PI), fixed, and measured for viability by flow cytometry. Viability was determined by the percentage of cells negative for both AV and PI. This experiment used 6 HCV-positive and 5 HCV-negative patient sera. Shown are the mean values ± SEM.