AC/S1P promotes Leptomycin B sensitive export of nuclear PTEN.

<p>PPC1 cells transfected with WT-PTEN were infected with Ad-GFP or Ad-AC for 48 hours in the presence of EtOH (NT) or 100 nM Leptomycin B (LMB) for 24 hours. A) Cells were immunostained for PTEN (red) and nuclei (blue). B) The percentage of cells from (A) which had nuclear PTEN in each treatment. C) Nuclear fractions from the indicated treatments were isolated and evaluated for presence of PTEN with Histone H3 as a nuclear loading control and absence of β-tubulin to indicate purity of the nuclear sample. D) PPC1 cells transfected with WT-PTEN were treated with 100nM LMB for 24 hours and 500nM S1P or PBS for 2 hours prior to fixation and immunostaining for PTEN (red) and nuclei (blue). E) The percentage of cells from (D) which had nuclear PTEN. F) Nuclear fractions from the indicated treatments were isolated and evaluated for presence of PTEN with Histone H3 as a nuclear loading control and absence of β-tubulin to indicate purity of the nuclear sample. One way ANOVA with Bonferroni correction, *p<.05**p<.01.