Expression of Ephrin-B1 in obese adipose tissue.

<p>A, Tissue distribution of Ephrin-B1 mRNA. Experiments were conducted in C57BL/6N mice under 12 hrs-fasting state at 12 weeks of age. B, Changes in adipose Ephrin-B1 mRNA level under high-fat/high-sucrose (HF/HS) diet. C57BL/6N mice were fed normal chaw diet (Cont) or HF/HS from 8 to 16 weeks of age. n=3 for each group. C, Adipose Ephrin-B1 mRNA levels in obese model mice. Ephrin-B1 mRNA level in WAT was examined in C57BL/6N (B6) and ob/ob (ob) mice at 8 and 16 weeks of age, respectively. n=6 for each group. D, Changes in adipose Ephrin-B1 protein level in mice of the obese model. Immunoblotting was performed using WAT of 16-week-old B6 and ob mice. Relative protein level (Ephrin-B1/α-tubulin) was calculated by densitometry. n=6 for each group. E, Ephrin-B1 mRNA level in fractionated WAT. WAT of 16-week-old B6 and ob mice was separated into mature adipocytes fraction (MAF) and stromal vascular fraction (SVF) as described in Materials and Methods section. n=5-6 for each group. <i>EfnB1</i> and EFNB1, Ephrin-B1; WAT, white adipose tissue; BAT, brown adipose tissue; DIO, diet-induced obesity; B6, C57BL/6J mice; ob, <i>ob/ob</i> mice; MAF, mature adipocytes fraction; SVF, stromal vascular fraction. Values are mean±SD. *<i>P</i><0.05; **<i>P</i><0.01; ***<i>P</i><0.001.