<i>In vitro</i> methylation silences <i>Foxl2</i> promoter reporter activity in NIH3T3 cells.

<p>The -432/+7 murine <i>Foxl2</i>-luciferase reporter in pCpGL-Basic was treated <i>in </i><i>vitro</i> with A) <i>M</i>.<i>Sss</i>I or B) M.<i>Hha</i>I. Mock methylated plasmid was exposed to the identical treatment but without enzyme. Plasmids were transfected in triplicate into NIH3T3 cells. For A (n=6) and B (n=4), the data reflect the means of independent experiments (+SEM) and are presented with empty vector activity set to 1. Data were analyzed by one-way ANOVA followed by Tukey <i>post-hoc</i> tests. Bars with different letters were statistically different, whereas those sharing letters did not differ.