<p><b>A</b>. Effects after 30 min incubation with the growth factor in control condition or with pretreatments with CPZ, SB431542, or SB203580, all for 1 h or DPI for 2 h. <b>B</b>. Equivalent results when the incubation was continued for 16 h after addition of TGF-β. Summary histograms of DCF fluorescein intensity depicting averages and standard error fold changes from the control values of three independent experiments are also shown.</p
<p>Fibroblasts were treated with TGF-β1 [10 ng/ml], PFD [10<sup>−3</sup> mol/l] or the combination o...
<p>(A) Isolated, unlabeled Teffs, without Tregs, were cultured in the indicated conditions with exog...
<p>Cells were incubated with10 μM dichlorodihydrofluorescein diacetate and ROS accumulation was esti...
<p>HCFs were exposed to TGF-β1 and relative changes in intracellular calcium were evaluated based on...
<p>A. Raw DCF fluorescence. B. Data normalized to basal levels. Cells were treated with 100 µM H<sub...
<p>Since DCF was used as a standard, intracellular ROS level is expressed as DCF equivalent (nM). Ea...
Cells were treated with 10 nM DEX for 6 h or 10 ng/ml TNF-α for 24 h. (A) Measurement of ROS generat...
<p><b>A</b>. Time course graph displayed a time-dependent increase of DCF fluorescence intensity in ...
<p>(A) Cells were pretreated with HG (30 mM glucose) for 72 hours and then incubated with bFGF (b, 1...
<p><b>A</b>. p-SMAD2 levels 30 min after introduction of TGF-β1 with or without prior addition of NA...
<p>Fura-2AM-loaded HCF were monitored for changes in [Ca<sup>2+</sup>]<sub>i</sub> using single cell...
<p>(A) Cells were incubated with bFGF (b, 100 ng/mL), JNK inhibitor SP600125 (SP, 25 µM) or bFGF tog...
<p>(1)–Example of reaction rate constant determination for DCF formation when DCFH reacts with ROS. ...
<p>(A) FC (1–100 µM) concentration-dependently increased ROS generation in the CEC. (B) The ROS gene...
<p>Jurkat cells (1 × 10<sup>6</sup>/well) were exposed to DHA (0, 10, 20, 40, 80 μM) or DHA/HTF for ...
<p>Fibroblasts were treated with TGF-β1 [10 ng/ml], PFD [10<sup>−3</sup> mol/l] or the combination o...
<p>(A) Isolated, unlabeled Teffs, without Tregs, were cultured in the indicated conditions with exog...
<p>Cells were incubated with10 μM dichlorodihydrofluorescein diacetate and ROS accumulation was esti...
<p>HCFs were exposed to TGF-β1 and relative changes in intracellular calcium were evaluated based on...
<p>A. Raw DCF fluorescence. B. Data normalized to basal levels. Cells were treated with 100 µM H<sub...
<p>Since DCF was used as a standard, intracellular ROS level is expressed as DCF equivalent (nM). Ea...
Cells were treated with 10 nM DEX for 6 h or 10 ng/ml TNF-α for 24 h. (A) Measurement of ROS generat...
<p><b>A</b>. Time course graph displayed a time-dependent increase of DCF fluorescence intensity in ...
<p>(A) Cells were pretreated with HG (30 mM glucose) for 72 hours and then incubated with bFGF (b, 1...
<p><b>A</b>. p-SMAD2 levels 30 min after introduction of TGF-β1 with or without prior addition of NA...
<p>Fura-2AM-loaded HCF were monitored for changes in [Ca<sup>2+</sup>]<sub>i</sub> using single cell...
<p>(A) Cells were incubated with bFGF (b, 100 ng/mL), JNK inhibitor SP600125 (SP, 25 µM) or bFGF tog...
<p>(1)–Example of reaction rate constant determination for DCF formation when DCFH reacts with ROS. ...
<p>(A) FC (1–100 µM) concentration-dependently increased ROS generation in the CEC. (B) The ROS gene...
<p>Jurkat cells (1 × 10<sup>6</sup>/well) were exposed to DHA (0, 10, 20, 40, 80 μM) or DHA/HTF for ...
<p>Fibroblasts were treated with TGF-β1 [10 ng/ml], PFD [10<sup>−3</sup> mol/l] or the combination o...
<p>(A) Isolated, unlabeled Teffs, without Tregs, were cultured in the indicated conditions with exog...
<p>Cells were incubated with10 μM dichlorodihydrofluorescein diacetate and ROS accumulation was esti...