Effect of inhibition of TRPV1 activity and ROS generation on the early and late p38 phosphorylation response to TGF-β1.

<p><b>A</b>. <b>Left</b>: Cells were pre-incubated for 15 min with 10µM CPZ, NAC, SB431542 (SB4) or for 2 h with DPI prior to exposure to TGF-β1 for 1 h with or without inhibitors. Cell lysates were Western blotted for p-p38 and p38. All inhibitors reduced p38 activation. <b>Right</b>: equivalent results in cells transfected with TRPV1 siRNA. <b>B</b>. <b>Left and Right</b>: Similar to A, but TGF-β1 treatment was extended to 16 h. Results shown are representative of three independent experiments performed.