Reduction of phosphorylated Akt by angiotensin II.

<p>(A) Representative Western blot analysis of phosphorylated Akt (p-Akt), total Akt, phosphorylated ERK_1,2 (p-ERK) and total ERK_1,2 in extracts from cells incubated 8 h in adipogenic medium without (-) or with (+) 1.5×10⁻⁵ M angiotensin II (AII) during the last 2 h. (B) p-Akt/Akt ratios determined from Western blots in the conditions indicated in A and expressed relative to the value in the corresponding controls maintained in adipogenic medium without angiotensin II. The mean pAkt/Akt for the controls in these experiments was 1.56±0.27 (n = 5 donors). (C) The Western blots illustrate phosphorylated Akt (p-Akt), total Akt, phosphorylated ERK_1,2 (p-ERK) and total ERK_1,2 in extracts from cells maintained in basal culture conditions and treated without (−) or with (+) angiotensin II during 2 h. (D) The p-Akt/Akt ratios were determined from Western blots and normalized with respect to the corresponding controls in the absence of angiotensin II. The mean pAkt/Akt value for the control condition in these experiments was 0.43±0.11 (n = 8 donors). Blots in part A were overexposed to enhance low chemoluminescent signals for phosphoproteins at this time point. The symbol * denotes p<0.05.