Methylation changes in <i>gria2</i> 5’ region in response to KA induced epileptiform activity.

<p>(A) Physical map of the <i>gria2</i> 5’ regulatory region and promoter region. CpG sites are marked by balloons and transcription factor predictions in the analyzed 5’ region are indicated above the physical map. (B) State of methylation of CpG sites in the proximal promoter of the <i>gria2</i> and the 5’ region in control and KA treated hippocampal slices (n=3 technical replicates); CpG 32 and 34 were not analyzed due to sequence restrictions. (C) Methylation differences between control and KA treated slices derived from the same mouse (n=4 technical replicates for each individual mouse) immediately after KA treatment for two hours, and (D) 1 week after removal of the drug (n=4 technical replicates, SD indicate technical errors). Inter-individual differences are apparent between littermate mice (Mouse I, II, III) and between mice from different litters (mouse IV, V) (E) <i>Gria2</i> mRNA expression levels in control and KA treated slices as measured by qPCR immediately after KA treatment (2 hours, n=5) and 1 week after removal of the drug (1 week, n=9). <i>Gria2</i> mRNA levels were normalized to TBP/GAPDH expression based on NormFinder. * p<0.05 ** p<0.01 *** p<0.005 as determined by Mann-Whitney U-test.