p53-dependent upregulation of Jagged1 expression induced by AngII.

<p>(<b>A</b>) Expression of Jagged1 and Dll-4 after AngII stimulation. Cultured CMVECs were stimulated with AngII (1 µM) for the indicated times. Jagged1 and Dll-4 expressions were detected by Western blotting. β-Actin was used as a loading control. Representative immunoblots from 3 independent experiments are shown. (<b>B</b>) Effects of p53 inhibitor on Jagged1 expression in CMVECs. CMVECs were pretreated with PFT-α or vehicle (DMSO) for 30 min and then stimulated with AngII (1 µM) or Ang II plus PFT-α for 18 hours. CMVECs treated with DMSO but without AngII served as the control (DMSO). Jagged1 expression was detected by Western blotting. β-Actin was used as a loading control. Representative immunoblots are shown. The expression of Jagged1 was quantified as folds of β-actin. Data are expressed as mean ±S.E.M. obtained from 3 independent experiments. (<b>C</b>) Effects of p53 inhibitor on Jagged1 expression in hearts of mice. Mice were treated by the method described under <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076529#pone-0076529-g003" target="_blank">Figure 3</a>. LV tissues were subjected to Western blot analysis for Jagged1 expression. β-Actin expression served as a loading control. Representative immunoblots are shown. The expression of Jagged1 was quantified as folds of β-actin. Data are expressed as mean ±S.E.M. obtained from 6 mice. (<b>D</b>) Effects of p53 inhibitor on RBPj –luc activity. HUVECs were transfected with rRBPj-Luc construct together with β-gal expression plasmid for 48h, after 30 min of PFT-α pretreatment, the cells were treated with Ang II for 24 hour and subjected for Luc assays. PFT-α: p53 inhibitor. Data are expressed as mean ±S.E.M. obtained from 3 independent experiments. ^*<i>p</i> < 0.05 ^**<i>p</i> < 0.01 <i>vs</i> DMSO or Saline; ^#<i>p</i> < 0.05 ^{# #}<i>p</i> < 0.01 <i>vs</i> AngII+DMSO.