Pathogenomic analysis of <i>Leptospira interrogans</i> serovar Lai strain 556021 to identify virulence related genes.

<p>(<b>A</b>) Schematic of phylogenetic relatedness of “Pathogenic” (P), “Intermediate” (I) and “Saprophytic” (S) members of the genus <i>Leptospira</i>. (<b>B</b>) Workflow to identify putative virulence-associated genes. Asterisk denotes a hypothetical position in which a SNV has been identified (<b>C</b>) Genomic Locations of SNPs and PF07598 paralogs in the reference genome of <i>L. interrogans</i> serovar Lai strain 56601. Each concentric circle represents genomic data and is numbered from the outermost to the innermost circle. The outermost circles represent the predicted CDS on the + and − strands, respectively, colored by functional role categories (see key). The following circle descriptions apply to chromosome I. The third circle notes the location of predicted prophage regions (olive) and the LPS region (slate). The fourth circle indicates those CDS found to have non-synonymous amino acid substitutions (black) as well as the location of CDS annotated as “transposase” in Genbank (salmon). The fifth circle represents the location of the 12 PF07598 family members (blue). The innermost circle denotes atypical regions (χ² value). For chromosome II, the outermost and innermost circles are the same as for chromosome I; however, the third circle notes the location of transposases (salmon), while the fourth circle indicates the location of the CDS found to have non-synonymous amino acid substitutions (black).