Effect of THI-56 on the expression levels of iNOS/NO and HMGB1 in LPS-stimulated macrophages.

<p>Cells were treated with THI-56 (10, 25, 50, 100 and 200 µM) for 24 h, and then the MTT assay (A) was performed as described in the Materials and Methods. Cells were treated with LPS (1 µg/ml) with and without THI-56 (1, 10, 25 µM) for 16 h. After incubation, the cells were lysed for iNOS detection (B). Culture medium was also collected for NO measurements (C) and HMGB1 analysis (D) as described in the Materials and Methods. The data are presented as the means ± SEM of three independent experiments. One-way analysis of variance followed by the Newman-Keuls test was used to compare the means of multiple groups (significant compared with the control, **<i>P</i> < 0.01; significant compared with LPS,^††<i>P</i> < 0.01).