Mice lacking the TGFβ-activating integrin αvβ8 on DCs demonstrate an enhanced IL-13 production in LILP CD4+ T-cells.

<p>(<i>A</i>) IL-13 and (<i>B</i>) IFNγ cytokine levels from <i>T. muris</i> E/S antigen-stimulated mLN cells from control and <i>Itgb8 (CD11c-Cre)</i> mice at different time-points post-infection ( p.i.) with a chronic dose of <i>T. muris</i> eggs, determined via cytometric bead array/ELISA. Data (n = 5–6 mice per group) are from three or more independent experiments. (<i>C</i>) Analysis of intracellular IL-13 and IFNγ expression in CD4+ T-cells isolated from control and <i>Itgb8 (CD11c-Cre)</i> mice at day 3 p.i. with a chronic dose of <i>T. muris</i> eggs. Representative flow cytometry plots and mean data are displayed. Data (n = 5–6 mice per group mice per group) are from three independent experiments. (<i>D</i>) Representative flow cytometry plots for IL-13 and IFNγ from LILP CD4+ T-cells at day 3 p.i. and (<i>E</i>) Serum antigen specific IgG1 and IgG2a levels at day 21–23 p.i. from control and anti-TGFβ antibody-treated C57BL/6 mice infected with a chronic dose of <i>T. muris</i> eggs. Data (n = 7–9 mice per group) are from two independent experiments performed. *, P<0.05; **, P<0.01; or ***, P<0.005, N.S., not significant via Kruskal–Wallis (A and B) and Student's <i>t</i>-test (C and E) for the indicated comparisons between groups, error bars represent SE of means.