Major metabolic pathways for the metabolites that are significantly altered in urine and serum from <i>ob</i>/<i>ob</i> mice compared to lean controls, observed by ¹H NMR spectroscopy.

<p>The pathways were analyzed on the basis of the KEGG pathway database (<a href="http://www.genome.jp/kegg/pathway.html" target="_blank">http://www.genome.jp/kegg/pathway.html</a>). The levels of the identified metabolites that were elevated and depleted in obese mice, compared to lean mice, are color coded as red and blue, respectively, and the metabolites in gray were not identified in our study. Direction of change is shown for all obese mice including males and females. For the direction of change based on gender, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075998#pone-0075998-t002" target="_blank">Tables 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075998#pone-0075998-t003" target="_blank">3</a>. The metabolites with asterisk are shown several times in this diagram to avoid the complexity of the pathways. The metabolites with sharp indicate the significant ones in male or female group only. Solid and dashed arrows indicate the single and multiple steps involved, respectively, between two metabolites.