MiR-199a-5p-mediated VEGF down-regulation in ADSCs.
- Guangfeng Chen (466409)
- Xiujuan Shi (466410)
- Chen Sun (177008)
- Min Li (12799)
- Qing Zhou (52712)
- Chen Zhang (66790)
- Jun Huang (127297)
- Yu Qiu (143428)
- Xiangyi Wen (466411)
- Yan Zhang (8098)
- Yushan Zhang (466412)
- Shuzhang Yang (42190)
- Lixia Lu (466413)
- Jieping Zhang (466414)
- Qionglan Yuan (466415)
- Jianwei Lu (432174)
- Guotong Xu (466416)
- Yunyun Xue (466417)
- Zibing Jin (466418)
- Cizhong Jiang (4119)
- Ming Ying (118264)
- Xiaoqing Liu (196900)
Publication date
October 2013
DOIAbstract
<p>(A) Targetscan indicates that miR-199a-5p binds the 3′UTR of VEGF mRNA; (B) Lentiviral infection efficiency was evaluated by ZsGreen intensity; (C) Dual-luciferase reporter assay indicated that miR-199a-5p interacted with the 3′UTR of VEGF mRNA by using lentiviral infection (n = 3); (D) qRT-PCR assay indicated that the lentiviruses overexpressed miR-199a-5p (n = 3); (E) qRT-PCR assay indicated that miR-199a-5p overexpression in ADSCs reduced VEGF mRNA levels by approximately 42% (n = 3). (F) ELISA assay indicated that miR-199a-5p overexpression in ADSCs reduced VEGF protein levels by approximately 16% (n = 5). The values were mean ± SEM (n = 5). **<i>P</i><0.01.</p
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