The appearance of immune cells in the ovaries of neonatal mice.

<p>(A–C) The ovaries of MRL/MpJ mice at postnatal day 0. Immunohistochemical analysis of F4/80 (macrophage marker, panel A), B220 (B cell marker, panel B), and CD3 (T cell marker, panel C). A few F4/80- and B220-positive cells (panels A and B), but no CD3-positive cells (panel C), are observed in the ovaries of the MRL/MpJ mice. The insets show a higher magnification of the boxed area in panels A and B. In panel C, the inset shows the spleen section at postnatal day 0 as a positive control. Bars in panels: 100 µm. Bars in insets: 10 µm. (D) Number of immune cells in the ovaries at postnatal day 0. Data represent the mean ± SEM (n = 3–6 in each group). Significant differences were analyzed using the Mann-Whitney <i>U</i> test. **: <i>P</i> < 0.01.